CALLUS INDUCTION AND SHOOT REGENERATION OF IN VITRO RICE VAR. FATMAWATI
To increase domestic and international demand of Kaemferia galanga makes this plantpotentially develop. Is traditionally used to keep the body warm, as analgetic and expectorant. In the attemp of providing adequately and qualitatively uniformed supply, in vitro experiment has been conducted at BB-Biogen (IndonesianCenter for Agricultural Biotechnology and Genetik Resources Research and Development).The selected rhizomes was used as explant. The experiment was orthogonally arranged consisting of MS vitamin and B5, and BA ( 0, 3 and 5 mg/l) and thidiazuron 0,1 mg/l. This experiment comprised three activities, they were shoot initiation, shoot multiplication and acclimatization. The result showed that MS + BA 3 mg/l + thidiazuron 0,2 mg/l could induce shoot formation. From the applied media, it was shown that the addition of MS vitamin at the MS basic media and BA 3 and 5 mg/l added with thidiazuron could result the most optimum shoot, leaves and roots and was not significantly different from the addition of B5 vitamin at basic media of MS + BA 3 and 5 mg/l, 6.9 shoot was averagely produced in this media. The shoot could generate such an adequate number of root that it could be directly acclimatized. The acclimatized plantlet in the green house uses the mixture of soil and manure with the ratio of 1:1 can optimally grow.
Bagi yang ingin mengetahui kultur jaringan lebih mendalam bisa menghubungi kami di BB-Biogen Bogor Jl. Tentara Pelajar No3A.Bogor Kami siap berbagi ilmu dengan anda Trimakasih
Buku pegangan untuk peneliti pemula dan mahasiswa
Welcome to my blog.
Let us not just talk about our future. Let's do a little thing to this world. Because the world is not ours, but belongs to our descendants. Let's do something to keep this tiny world preserved. This will make the life of the next generation easier. Plant is our mother where we suck the milk of life. Killing plant is more horrible than matricide. It kills our source of air. The natural deterioration as a result of human activity should be balanced with our effort to preserve plant. Let's work hand in hand to keep our nature smile forever.
Born in Temanggung,Central Java, I was raised among the elementary school teacher. Since my childhood, I was told the story about the story of "Cucumber Princes". I was indebted to the story that, for me, plants are inseparable parts of our life. We cannot live longer without plant preserved and reserved for us. Therefore, the act of preserving is like the act of keeping ourselves alive.
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